After linking that ligand to the well-established Toyopearl rigid methacrylic polymer backbone, we obtained a stable resin with high dynamic binding capacity (DBC) at fast flow rates. Our ligand is a genetically optimized recombinant protein L with high chemical stability. Tosoh Bioscience has developed Toyopearl AF-rProtein L-650F as the best-in-class affinity resin for antibody fragment capture. Protein L is a surface protein of Peptostreptococcus magnus, which binds the κ-light-chain of antibodies present in most common fragments. As antibody fragments do not have an Fc-region, chromatography experts cannot use protein A affinity and must select protein L as a ligand for fragment capture. Downstream processing, however, can be challenging. They also can be produced in prokaryotic cells (such as Escherichia coli), a cell line more economic than mammalian expression systems. Recombinant-based antibody fragments can be modified to meet specific needs of affinity, avidity, valence, and action mode. Upstream processing for antibody fragments is easier than it is for standard antibodies. These drugs offer several therapeutic advantages over conventional monoclonal antibodies. Antibody fragments are either separate functional subunits of antibodies or recombinant molecules, which, just like antibodies, are composed of immunoglobulin domains. Some of the latest promising biopharmaceutical drug substances are antibody fragments. Tosoh Bioscience’s SkillPak 1-mL and 5-mL prepacked columns
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